Protein_Domain
Part:BBa_K1023008:Design
Designed by: Michiel Karrenbelt Group: iGEM13_Wageningen_UR (2013-09-17)
Malonyl-CoA:ACP acyltransferase The Malonyl-CoA:ACP acyltransferase (MAT) domain is derived from the lovB gene of Aspergillus terreus, it is the second domain of the naturally occurring iterative polyketide synthase, and it is codon optimized for Aspergillus niger. LovB is involved in the production of the first intermediate in the lovastatin pathway, dihydromonacolin L. The MAT domain is able to select molecules used for extension of the substrate and to transfer them in the form of malonic esters.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 712
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 244
Illegal SapI.rc site found at 1218
The MAT domain is codon optimized for Aspergillus niger, the original sequence is derived form the lovB gene of Aspergillus terreus.
Design Notes
Source
Aspergillus terreus